Vector Tick Transmission Model of Spotted Fever Rickettsiosis

Tais Saito, Jeremy Bechelli, Claire Smalley, Shahid Karim, David Walker

Research output: Contribution to journalArticle

Abstract

Many aspects of rickettsial infections have been characterized, including pathogenic and immune pathways and mechanisms of rickettsial survival within the vertebrate host and tick vector. However, very few studies are focused on the complex pathogen–vector–host interactions during tick feeding. Therefore, our objective was to develop a tick transmission model of the spotted fever group of rickettsial infections to study the initial events in disease development. The most appropriate strain of mouse was identified for evaluation as a transmission model, and the course of infection, bacterial levels, histopathologic changes, and antibody response during tick transmission in mice infested with Amblyomma maculatum ticks carrying the emerging pathogen, Rickettia parkeri, were studied. Results showed distinct clinical signs in C3H/HeN mice infected intravenously, leading to selection of this mouse strain for tick transmission studies. Active infection of animals was observed after tick vector transmission. The bacteria disseminated systemically and spread to several organs at 24 hours after tick attachment, with peak bacterial load at day 6 after tick attachment. Skin, lung, and liver showed the greatest pathologic changes, with inflammatory cellular infiltration and necrosis. These findings indicate the feasibility of using murine infection with R. parkeri by A. maculatum tick transmission as a model to study different aspects of the spotted fever group of rickettsial disease establishment.

LanguageEnglish (US)
Pages115-123
Number of pages9
JournalAmerican Journal of Pathology
Volume189
Issue number1
DOIs
StatePublished - Jan 1 2019

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Ticks
Fever
Infection
Host-Pathogen Interactions
Inbred C3H Mouse
Bacterial Load
Bacterial Infections
Antibody Formation
Vertebrates
Necrosis
Bacteria
Lung
Skin
Liver

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Vector Tick Transmission Model of Spotted Fever Rickettsiosis. / Saito, Tais; Bechelli, Jeremy; Smalley, Claire; Karim, Shahid; Walker, David.

In: American Journal of Pathology, Vol. 189, No. 1, 01.01.2019, p. 115-123.

Research output: Contribution to journalArticle

Saito, Tais ; Bechelli, Jeremy ; Smalley, Claire ; Karim, Shahid ; Walker, David. / Vector Tick Transmission Model of Spotted Fever Rickettsiosis. In: American Journal of Pathology. 2019 ; Vol. 189, No. 1. pp. 115-123.
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