Abstract
Most analyses of genital immunity to herpes simplex virus type 2 (HSV-2) have been performed in females, consequently immune protection of the male genital epithelium is incompletely understood. We developed a model of male genital HSV-2 infection resulting from intrarectal inoculation of guinea pigs. Vesicular lesions developed transiently on the perineum and foreskin concurrent with acute virus shedding. Virus shedding and recurrent genital lesions were also detected after establishment of a latent infection. Analysis of perineum and foreskin RNA detected transcripts for IFNγ, proinflammatory and regulatory cytokines, and for genes involved in migration and regulation of leukocytes. HSV-specific T cells were detected in lymphoid and genital tissues after resolution of the primary infection whereas virus-specific antibody secreting cells were detected only in lymphoid tissue. Taken together, the ability to quantify pathogenesis and local immunity in this guinea pig model represent an important advance towards understanding immunity to HSV-2 in males
| Original language | English (US) |
|---|---|
| Pages (from-to) | 180-188 |
| Number of pages | 9 |
| Journal | Virology |
| Volume | 526 |
| DOIs | |
| State | Published - Jan 2 2019 |
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Keywords
- Foreskin
- Gene expression
- HSV-2
- IFNγ
- Male guinea pig
- Microarray
- Perineum
ASJC Scopus subject areas
- Virology
Cite this
Development of disease and immunity at the genital epithelium following intrarectal inoculation of male guinea pigs with herpes simplex virus type 2. / Bourne, Nigel; Banasik, Brianne N.; Perry, Clarice L.; Miller, Aaron L.; White, Mellodee; Pyles, Richard; Milligan, Gregg.
In: Virology, Vol. 526, 02.01.2019, p. 180-188.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Development of disease and immunity at the genital epithelium following intrarectal inoculation of male guinea pigs with herpes simplex virus type 2
AU - Bourne, Nigel
AU - Banasik, Brianne N.
AU - Perry, Clarice L.
AU - Miller, Aaron L.
AU - White, Mellodee
AU - Pyles, Richard
AU - Milligan, Gregg
PY - 2019/1/2
Y1 - 2019/1/2
N2 - Most analyses of genital immunity to herpes simplex virus type 2 (HSV-2) have been performed in females, consequently immune protection of the male genital epithelium is incompletely understood. We developed a model of male genital HSV-2 infection resulting from intrarectal inoculation of guinea pigs. Vesicular lesions developed transiently on the perineum and foreskin concurrent with acute virus shedding. Virus shedding and recurrent genital lesions were also detected after establishment of a latent infection. Analysis of perineum and foreskin RNA detected transcripts for IFNγ, proinflammatory and regulatory cytokines, and for genes involved in migration and regulation of leukocytes. HSV-specific T cells were detected in lymphoid and genital tissues after resolution of the primary infection whereas virus-specific antibody secreting cells were detected only in lymphoid tissue. Taken together, the ability to quantify pathogenesis and local immunity in this guinea pig model represent an important advance towards understanding immunity to HSV-2 in males
AB - Most analyses of genital immunity to herpes simplex virus type 2 (HSV-2) have been performed in females, consequently immune protection of the male genital epithelium is incompletely understood. We developed a model of male genital HSV-2 infection resulting from intrarectal inoculation of guinea pigs. Vesicular lesions developed transiently on the perineum and foreskin concurrent with acute virus shedding. Virus shedding and recurrent genital lesions were also detected after establishment of a latent infection. Analysis of perineum and foreskin RNA detected transcripts for IFNγ, proinflammatory and regulatory cytokines, and for genes involved in migration and regulation of leukocytes. HSV-specific T cells were detected in lymphoid and genital tissues after resolution of the primary infection whereas virus-specific antibody secreting cells were detected only in lymphoid tissue. Taken together, the ability to quantify pathogenesis and local immunity in this guinea pig model represent an important advance towards understanding immunity to HSV-2 in males
KW - Foreskin
KW - Gene expression
KW - HSV-2
KW - IFNγ
KW - Male guinea pig
KW - Microarray
KW - Perineum
UR - http://www.scopus.com/inward/record.url?scp=85056159786&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85056159786&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2018.10.023
DO - 10.1016/j.virol.2018.10.023
M3 - Article
VL - 526
SP - 180
EP - 188
JO - Virology
JF - Virology
SN - 0042-6822
ER -