An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope

Jing Jin, Michael Sherman, Daniel Chafets, Nuntana Dinglasan, Kai Lu, Tzong Hae Lee, Lars Anders Carlson, Marcus O. Muench, Graham Simmons

Research output: Contribution to journalArticle

Abstract

Background: Chikungunya virus (CHIKV) is the most common alphavirus infecting humans worldwide, causing acute and chronically debilitating arthralgia at a great economic expense. Methodology/Principal findings: To facilitate our study of CHIKV, we generated a mCherry tagged replication-competent chimeric virus, CHIKV 37997-mCherry. Single particle cryoEM demonstrated icosahedral organization of the chimeric virus and the display of mCherry proteins on virus surface. CHIKV 37997-mCherry is attenuated in both IFNαR knockout and wild-type mice. Strong anti-CHIKV and anti-mCherry antibody responses were induced in CHIKV 37997-mCherry infected mice. Conclusions/Significance: Our work suggests that chimeric alphaviruses displaying foreign antigen can serve as vaccines against both aphaviruses and other pathogens and diseases.

LanguageEnglish (US)
Article numbere0006693
JournalPLoS Neglected Tropical Diseases
Volume12
Issue number7
DOIs
StatePublished - Jul 31 2018

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Chikungunya virus
Alphavirus
Viruses
Arthralgia
Knockout Mice
Antibody Formation
Anti-Idiotypic Antibodies
Vaccines
Economics
Organizations
Antigens

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope. / Jin, Jing; Sherman, Michael; Chafets, Daniel; Dinglasan, Nuntana; Lu, Kai; Lee, Tzong Hae; Carlson, Lars Anders; Muench, Marcus O.; Simmons, Graham.

In: PLoS Neglected Tropical Diseases, Vol. 12, No. 7, e0006693, 31.07.2018.

Research output: Contribution to journalArticle

Jin, J, Sherman, M, Chafets, D, Dinglasan, N, Lu, K, Lee, TH, Carlson, LA, Muench, MO & Simmons, G 2018, 'An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope' PLoS Neglected Tropical Diseases, vol. 12, no. 7, e0006693. https://doi.org/10.1371/journal.pntd.0006693
Jin, Jing ; Sherman, Michael ; Chafets, Daniel ; Dinglasan, Nuntana ; Lu, Kai ; Lee, Tzong Hae ; Carlson, Lars Anders ; Muench, Marcus O. ; Simmons, Graham. / An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope. In: PLoS Neglected Tropical Diseases. 2018 ; Vol. 12, No. 7.
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