Amniotic fluid exosome proteomic profile exhibits unique pathways of term and preterm labor

C. Luke Dixon, Samantha Sheller-Miller, George R. Saade, Stephen J. Fortunato, Andrew Lai, Carlos Palma, Dominic Guanzon, Carlos Salomon, Ramkumar Menon

Research output: Contribution to journalArticle

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Abstract

Our objective was to determine the amniotic fluid–derived exosomal proteomic profile in patients who had spontaneous preterm birth (PTB) or preterm premature rupture of membranes (pPROM) compared with those who delivered at term. A cross-sectional study of a retrospective cohort was used to quantify and determine the protein content of exosomes present in amniotic fluid, in PTB or pPROM, and normal term labor (TL) or term not in labor (TNIL) pregnancies. Exosomes were isolated by differential centrifugation and quantified using nanocrystals (Qdot) coupled to CD63 and placental alkaline phosphatase (PLAP) by fluorescence nanoparticle tracking analysis. The exosomal proteomic profile was identified by liquid chromatography–tandem mass spectrometry, and a small ion library was constructed to quantify the proteomic data by Sequential Window Acquisition of All Theoretical analysis. Ingenuity Pathway Analysis determined canonical pathways and biofunctions associated with dysregulated proteins. Amniotic fluid exosomes have similar shape and quantity regardless of the conditions; however, the PLAP/CD63 ratios for TL, PTB, and pPROM were significantly higher (;3.8-,;4.4-, and;3.5-fold, respectively) compared with TNIL. The PLAP/CD63 ratio was also significantly higher (;1.3-fold) in PTB compared with pPROM. Biological functions primarily indicated nonspecific inflammatory response regardless of condition, but unique profiles were also identified in cases (PTB and pPROM) compared with term. Amniotic fluid exosomes provide information specific to normal and abnormal parturition. Inflammatory marker enrichment and its uniqueness in term and preterm pregnancies support the value of exosomes in determining underlying physiology associated with term and preterm parturition.

LanguageEnglish (US)
Pages2229-2240
Number of pages12
JournalEndocrinology
Volume159
Issue number5
DOIs
StatePublished - May 1 2018

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Exosomes
Premature Obstetric Labor
Premature Birth
Amniotic Fluid
Proteomics
Nanoparticles
Parturition
Pregnancy
Tandem Mass Spectrometry
Centrifugation
Liquid Chromatography
Proteins
Cross-Sectional Studies
Fluorescence
Preterm Premature Rupture of the Membranes
Ions
placental alkaline phosphatase

ASJC Scopus subject areas

  • Endocrinology

Cite this

Dixon, C. L., Sheller-Miller, S., Saade, G. R., Fortunato, S. J., Lai, A., Palma, C., ... Menon, R. (2018). Amniotic fluid exosome proteomic profile exhibits unique pathways of term and preterm labor. Endocrinology, 159(5), 2229-2240. https://doi.org/10.1210/en.2018-00073

Amniotic fluid exosome proteomic profile exhibits unique pathways of term and preterm labor. / Dixon, C. Luke; Sheller-Miller, Samantha; Saade, George R.; Fortunato, Stephen J.; Lai, Andrew; Palma, Carlos; Guanzon, Dominic; Salomon, Carlos; Menon, Ramkumar.

In: Endocrinology, Vol. 159, No. 5, 01.05.2018, p. 2229-2240.

Research output: Contribution to journalArticle

Dixon, CL, Sheller-Miller, S, Saade, GR, Fortunato, SJ, Lai, A, Palma, C, Guanzon, D, Salomon, C & Menon, R 2018, 'Amniotic fluid exosome proteomic profile exhibits unique pathways of term and preterm labor' Endocrinology, vol. 159, no. 5, pp. 2229-2240. https://doi.org/10.1210/en.2018-00073
Dixon CL, Sheller-Miller S, Saade GR, Fortunato SJ, Lai A, Palma C et al. Amniotic fluid exosome proteomic profile exhibits unique pathways of term and preterm labor. Endocrinology. 2018 May 1;159(5):2229-2240. https://doi.org/10.1210/en.2018-00073
Dixon, C. Luke ; Sheller-Miller, Samantha ; Saade, George R. ; Fortunato, Stephen J. ; Lai, Andrew ; Palma, Carlos ; Guanzon, Dominic ; Salomon, Carlos ; Menon, Ramkumar. / Amniotic fluid exosome proteomic profile exhibits unique pathways of term and preterm labor. In: Endocrinology. 2018 ; Vol. 159, No. 5. pp. 2229-2240.
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