Abstract
Our objective was to determine the amniotic fluid–derived exosomal proteomic profile in patients who had spontaneous preterm birth (PTB) or preterm premature rupture of membranes (pPROM) compared with those who delivered at term. A cross-sectional study of a retrospective cohort was used to quantify and determine the protein content of exosomes present in amniotic fluid, in PTB or pPROM, and normal term labor (TL) or term not in labor (TNIL) pregnancies. Exosomes were isolated by differential centrifugation and quantified using nanocrystals (Qdot) coupled to CD63 and placental alkaline phosphatase (PLAP) by fluorescence nanoparticle tracking analysis. The exosomal proteomic profile was identified by liquid chromatography–tandem mass spectrometry, and a small ion library was constructed to quantify the proteomic data by Sequential Window Acquisition of All Theoretical analysis. Ingenuity Pathway Analysis determined canonical pathways and biofunctions associated with dysregulated proteins. Amniotic fluid exosomes have similar shape and quantity regardless of the conditions; however, the PLAP/CD63 ratios for TL, PTB, and pPROM were significantly higher (;3.8-,;4.4-, and;3.5-fold, respectively) compared with TNIL. The PLAP/CD63 ratio was also significantly higher (;1.3-fold) in PTB compared with pPROM. Biological functions primarily indicated nonspecific inflammatory response regardless of condition, but unique profiles were also identified in cases (PTB and pPROM) compared with term. Amniotic fluid exosomes provide information specific to normal and abnormal parturition. Inflammatory marker enrichment and its uniqueness in term and preterm pregnancies support the value of exosomes in determining underlying physiology associated with term and preterm parturition.
| Language | English (US) |
|---|---|
| Pages | 2229-2240 |
| Number of pages | 12 |
| Journal | Endocrinology |
| Volume | 159 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 1 2018 |
Fingerprint
ASJC Scopus subject areas
- Endocrinology
Cite this
Amniotic fluid exosome proteomic profile exhibits unique pathways of term and preterm labor. / Dixon, C. Luke; Sheller-Miller, Samantha; Saade, George; Fortunato, Stephen J.; Lai, Andrew; Palma, Carlos; Guanzon, Dominic; Salomon, Carlos; Menon, Ramkumar.
In: Endocrinology, Vol. 159, No. 5, 01.05.2018, p. 2229-2240.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Amniotic fluid exosome proteomic profile exhibits unique pathways of term and preterm labor
AU - Dixon, C. Luke
AU - Sheller-Miller, Samantha
AU - Saade, George
AU - Fortunato, Stephen J.
AU - Lai, Andrew
AU - Palma, Carlos
AU - Guanzon, Dominic
AU - Salomon, Carlos
AU - Menon, Ramkumar
PY - 2018/5/1
Y1 - 2018/5/1
N2 - Our objective was to determine the amniotic fluid–derived exosomal proteomic profile in patients who had spontaneous preterm birth (PTB) or preterm premature rupture of membranes (pPROM) compared with those who delivered at term. A cross-sectional study of a retrospective cohort was used to quantify and determine the protein content of exosomes present in amniotic fluid, in PTB or pPROM, and normal term labor (TL) or term not in labor (TNIL) pregnancies. Exosomes were isolated by differential centrifugation and quantified using nanocrystals (Qdot) coupled to CD63 and placental alkaline phosphatase (PLAP) by fluorescence nanoparticle tracking analysis. The exosomal proteomic profile was identified by liquid chromatography–tandem mass spectrometry, and a small ion library was constructed to quantify the proteomic data by Sequential Window Acquisition of All Theoretical analysis. Ingenuity Pathway Analysis determined canonical pathways and biofunctions associated with dysregulated proteins. Amniotic fluid exosomes have similar shape and quantity regardless of the conditions; however, the PLAP/CD63 ratios for TL, PTB, and pPROM were significantly higher (;3.8-,;4.4-, and;3.5-fold, respectively) compared with TNIL. The PLAP/CD63 ratio was also significantly higher (;1.3-fold) in PTB compared with pPROM. Biological functions primarily indicated nonspecific inflammatory response regardless of condition, but unique profiles were also identified in cases (PTB and pPROM) compared with term. Amniotic fluid exosomes provide information specific to normal and abnormal parturition. Inflammatory marker enrichment and its uniqueness in term and preterm pregnancies support the value of exosomes in determining underlying physiology associated with term and preterm parturition.
AB - Our objective was to determine the amniotic fluid–derived exosomal proteomic profile in patients who had spontaneous preterm birth (PTB) or preterm premature rupture of membranes (pPROM) compared with those who delivered at term. A cross-sectional study of a retrospective cohort was used to quantify and determine the protein content of exosomes present in amniotic fluid, in PTB or pPROM, and normal term labor (TL) or term not in labor (TNIL) pregnancies. Exosomes were isolated by differential centrifugation and quantified using nanocrystals (Qdot) coupled to CD63 and placental alkaline phosphatase (PLAP) by fluorescence nanoparticle tracking analysis. The exosomal proteomic profile was identified by liquid chromatography–tandem mass spectrometry, and a small ion library was constructed to quantify the proteomic data by Sequential Window Acquisition of All Theoretical analysis. Ingenuity Pathway Analysis determined canonical pathways and biofunctions associated with dysregulated proteins. Amniotic fluid exosomes have similar shape and quantity regardless of the conditions; however, the PLAP/CD63 ratios for TL, PTB, and pPROM were significantly higher (;3.8-,;4.4-, and;3.5-fold, respectively) compared with TNIL. The PLAP/CD63 ratio was also significantly higher (;1.3-fold) in PTB compared with pPROM. Biological functions primarily indicated nonspecific inflammatory response regardless of condition, but unique profiles were also identified in cases (PTB and pPROM) compared with term. Amniotic fluid exosomes provide information specific to normal and abnormal parturition. Inflammatory marker enrichment and its uniqueness in term and preterm pregnancies support the value of exosomes in determining underlying physiology associated with term and preterm parturition.
UR - http://www.scopus.com/inward/record.url?scp=85052101269&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85052101269&partnerID=8YFLogxK
U2 - 10.1210/en.2018-00073
DO - 10.1210/en.2018-00073
M3 - Article
VL - 159
SP - 2229
EP - 2240
JO - Endocrinology
T2 - Endocrinology
JF - Endocrinology
SN - 0013-7227
IS - 5
ER -